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KMID : 0880220170550090673
Journal of Microbiology
2017 Volume.55 No. 9 p.673 ~ p.677
Spirosoma metallicus sp. nov., isolated from an automobile air conditioning system
Lee Hyo-Sun

Kim Dong-Uk
Lee Su-Yeon
Park Soo-Yeon
Yoon Jung-Hoon
Park So-Yoon
Ka Jong-Ok
Abstract
A Gram-stain-negative and orangish yellow-pigmented bacterial strain, designated PR1014KT, was isolated from an automobile evaporator core collected in Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PR1014KT was related with the members of the genus Spirosoma (94.7?90.2%) and closely related with Spirosoma lacussanchae CPCC 100624T (94.7%), Spirosoma knui 15J8-12T (94.3%), and Spirosoma soli MIMBbqt12T (93.3%). The strain grew at 15?40¡ÆC (optimum, 25¡ÆC), pH 6.5?7.0 (optimum, 6.5) and 0?1% (w/v) NaCl (optimum, 0%). The predominant fatty acids were summed feature 3 (C16:1 ¥ø7c and/or C16:1 ¥ø6c), C16:0, iso-C15:0, C16:1 ¥ø5c, and iso-C17:0 3-OH. The major menaquinone was MK-7. The polar lipid profile of the strain indicated that the presence of one phosphatidylethanolamine, one unidentified aminolipid, two unidentified aminophospholipids, and three unidentified lipids. The DNA G+C content of the strain was 47.4 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain PR1014KT represents a novel species in the genus Spirosoma, for which the name Spirosoma metallicus sp. nov. (=KACC 17940T =NBRC 110792T) is proposed.
KEYWORD
Spirosoma, automobile air conditioning system, polyphasic taxonomy
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